However, TIRF classically suffers from interference illumination patterns such as fringes or rings inherent to the technique.
In our theoretical opinion and experience, the best way to generate the nicest TIRF images through a microscope objective is to spin in its back focal plane to average out all artifacts. By using a pair of fast galvanometers, iLas 2 TIRF system is able to spin several times during a single exposure time, even the shortest ones. This speed also allows artefact-free oblique illumination as well as high SNR wide field imaging using the same laser source. To go even further, TIRF/wide-field and multichannel-multi penetration depth acquisition are made possible.
Moreover, its optical design enables to simultaneously combine TIRF with FRAP and ablation.