iLas 2

The iLas 2 platform is a unique multi-application device that offers complete control over any laser illumination. Coupled to a wide-field or spinning disk confocal microscope, its evolutive design allows researchers to choose and simultaneously combine:

  • Azimuthal TIRF imaging
  • 3D TIRF – θscan
  • Single molecule imaging
  • Oblique illumination imaging
  • FRAP Photo-activation
The iLas 2 system can be declined in three versions:
  • iLas 2 FRAP (upright or inverted)
  • iLas 2 TIRF
  • iLas 2 TIRF/FRAP

Key features

  • Unmatched uniformity in TIRF and HILO
  • Calibrated angle and penetration depth
  • Simultaneous multichannel TIRF
  • FRAP on fly
  • Simultaneous multi-penetration depths at multiple wavelengths
  • Simultaneous TIRF & FRAP
  • 3D TIRF Super resolution

Modular V2.0 GATACA software

Improved interface and new functionnalities

Modular 360 TIRF:

  • Fast and easy calibration for your azimuthal TIRF experiment (up to 6 calibrations)
  • Easy switch between averaging modalities, 360 ellipse, arc or point TIRF
  • Very intuitive user interface for live navigation between Widefield, HILO or TIRF
  • Live fine tuning of your illumination settings (angle, power, depth, …)
  • Multicolor steaming for fast multicolor acquisition at same penetration depth
  • Simultaneous or sequential multicolor excitation and detection
  • Multicamera capability with different penetration depth
  • Theta-scan acquisition mode for axial super resolution (50nm resolution)

Modular TARGET for FRAP FLIP and ablation:

  • Easy and robust auto calibration
  • Simple ROI manager
  • On the fly FRAP/ablation with points, spirals or ROIs
  • Intuitive power control, adjustable repetition and thickness
  • Integrated FRAP and FLIP providing experimental pre-settings overview
  • 256 level image importation for precise patterning and complexes ROIs
Screeshot Gataca MOdular
MOdular screenshot
REAL TIME patterning using the 488 nm laser on a large sCMOS camera. 256 gray levels, 18mm FOV