Neural Stem Cells in a human forebrain organoids
Cerebral organoids are derived from human induced pluripotent stem cells. Those mini brains in 3D show multiple lumens that recapitulate the organization of the developing cortex. Neural stem cells (green) form a neuroepithelium that surround the lumens. Stem cells divisions occur mostly at the lumen border, with condensed DNA (blue) and mitotic spindle (red).
This picture was performed with a Gataca systems’ spinning disk using a 40X water immersion objective: it is a 3D mosaic of 3×3 fields of view, 17 stacks with a step size of two microns. Then the mosaic was reconstructed in 3D and a maximum projection was finally processed.