In order to go even further iLas 3 adds photo-ablation capabilities to iLas 2. Coupled to a wide-field or spinning disk confocal microscope, its evolutive design allows researchers to choose and simultaneously combine:
Azimuthal TIRF imaging
3D TIRF – θscan
Single molecule imaging
Oblique illumination imaging
FRAP Photo-activation
Photo-ablation
Key features
Unmatched uniformity in TIRF and HILO
Calibrated angle and penetration depth
Simultaneous multichannel TIRF
Simultaneous multi-penetration depths
Simultaneous TIRF & FRAP & Photo-ablation
Keep polarization state of each laser
Very large illumination spectrum
Modular V2.0 GATACA software
Improved interface and new functionnalities
Modular 360 TIRF:
Fast and easy calibration for your azimuthal TIRF experiment (up to 6 calibrations)
Easy switch between averaging modalities, 360 ellipse, arc or point TIRF
Very intuitive user interface for live navigation between Widefield, HILO or TIRF
Live fine tuning of your illumination settings (angle, power, depth, …)
Multicolor steaming for fast multicolor acquisition at same penetration depth
Simultaneous or sequential multicolor excitation and detection
Multicamera capability with different penetration depth
Theta-scan acquisition mode for axial super resolution (50nm resolution)
Modular TARGET for FRAP FLIP and ablation:
Easy and robust auto calibration
Simple ROI manager
On the fly FRAP/ablation with points, spirals or ROIs
Intuitive power control, adjustable repetition and thickness
Integrated FRAP and FLIP providing experimental pre-settings overview
256 level image importation for precise patterning and complexes ROIs
REAL TIME patterning using the 488 nm laser on a large sCMOS camera. 256 gray levels, 18mm FOV