However, TIRF classically suffer from interference illumination patterns such as fringes or rings, inherent to the technique.
In our theoretical opinion and experience, the best way to generate the nicest TIRF images through a microscope objective is to spin in its back focal plane to average out all artifacts. By using a pair of fast galvanometer iLas 2 TIRF system is able to spin several times a single exposure time, even the shortest ones. This speed also allows artefact-free oblique illumination as well as high SNR wide field imaging from the same laser source. To go even further TIRF/widefield and multichannel-multi penetration depth acquisition are made possible.